Composite

Part:BBa_K3440007:Design

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)


AiiA-Myc under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to show that AiiA can be expressed under constitutive promoter J23100. A myc tag was added for characterization. We used a moderate strength RBS (BBa_B0034)

Source

AiiA from Bacillus thuringiensis

References